human bc cell lines sw 780  (ATCC)

Journal: Journal of Cancer

Article Title: C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways

doi: 10.7150/jca.56993

Figure Lengend Snippet: Silencing of C19orf10 inhibited the migration and invasion of BC cells. (A-B) The mRNA (A) and protein (B) expression levels of C19orf10 were determined by qPCR and western blot assays, respectively. ** P < 0.01, *** P < 0.001. (C-D) Transwell assays were used to assess the migration and invasion of SW780 cells (C) and J82 (D) cells at 48 h after transfection of negative control or C19orf10-specific siRNA oligos. Representative images of cell migration and invasion in the transwell experiments are shown (C-D), and the relative cell motility was quantitated. n = 3 for each group; *** P < 0.001, compared with the control group.

Article Snippet: The following cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA): human BC cell lines SW780 (ATCC® CRL-2169), UMUC3 (ATCC® CRL-1749), 5637 (ATCC® HTB-9), T24 (ATCC® HTB-4), TCCSUP (ATCC® HTB-5TM), and J82 (ATCC® HTB-1), and the immortalized urothelial cell line SV-HUC-1 (ATCC® CRL-9520).

Techniques: Migration, Expressing, Western Blot, Transfection, Negative Control, Control

Journal: Journal of Cancer

Article Title: C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways

doi: 10.7150/jca.56993

Figure Lengend Snippet: Silencing of C19orf10 inhibited the proliferation and colony formation of BC cells. (A-D) SW780 and J82 cells were transfected with negative control or C19orf10-specific siRNA oligos, and cell proliferation and colony formation were assessed at 48 h after transfection. (A-B) The cell viability after silencing C19orf10 in SW780 (A) and J82 (B) cells was measured using the CCK-8 assay. * P < 0.05, ** P < 0.01, *** P < 0.001. (C)The cell proliferation rates of SW780 cells and J82 cells after silencing C19orf10 were assessed using a colony formation assay, and the number of cell colonies was normalized to that of the negative control group. n = 3 for each group; *** P < 0.001, compared with the control group. (D) Detection of cell proliferation by EdU incorporation assay. The relative proliferation rate was quantitated by calculating the percent of EdU-positive cells. * P < 0.05, *** P < 0.001.

Article Snippet: The following cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA): human BC cell lines SW780 (ATCC® CRL-2169), UMUC3 (ATCC® CRL-1749), 5637 (ATCC® HTB-9), T24 (ATCC® HTB-4), TCCSUP (ATCC® HTB-5TM), and J82 (ATCC® HTB-1), and the immortalized urothelial cell line SV-HUC-1 (ATCC® CRL-9520).

Techniques: Transfection, Negative Control, CCK-8 Assay, Colony Assay, Control

Journal: Journal of Cancer

Article Title: C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways

doi: 10.7150/jca.56993

Figure Lengend Snippet: Deficiency of C19orf10 inhibited the malignant growth of subcutaneous cancer xenografts in vivo . (A) The protein levels of C19orf10 in stable SW780 cells with infection of control or C19orf10-shRNA-expressing lentivirus were determined by western blot assays. (B) The infection efficiency of lentivirus in the above cells was observed under a fluorescence microscope. (C) Growth curves show the tumor sizes in nude mice inoculated with the indicated SW780 cell lines (NC, sh-C19orf10#1, and sh-C19orf10#3 cells) during an observation time of four weeks. n=5 per group; * P < 0.05, ** P < 0.01. (D-E) Mice were sacrificed at 4 weeks after tumor cells inoculation. The images of mice at end-point are shown (D), and the excised tumor tissues were images and weighed (E). * P < 0.05, ** P < 0.01. (F) Representative images of immunohistochemical staining (IHC) analysis of PCNA and Ki67 protein levels in tumor tissues. Scale bars, 100 µm.

Article Snippet: The following cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA): human BC cell lines SW780 (ATCC® CRL-2169), UMUC3 (ATCC® CRL-1749), 5637 (ATCC® HTB-9), T24 (ATCC® HTB-4), TCCSUP (ATCC® HTB-5TM), and J82 (ATCC® HTB-1), and the immortalized urothelial cell line SV-HUC-1 (ATCC® CRL-9520).

Techniques: In Vivo, Infection, Control, shRNA, Expressing, Western Blot, Fluorescence, Microscopy, Immunohistochemical staining, Staining

Journal: Journal of Cancer

Article Title: C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways

doi: 10.7150/jca.56993

Figure Lengend Snippet: C19orf10 promotes malignant behaviors and EMT of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathway. (A) Western blotting analysis of PI3K, p-AKT, total AKT, p21, and p27 expressions in control or C19orf10 siRNA-expressing SW780 and J82 cells. (B) Western blotting analysis of PI3K, p-AKT, AKT (pan), p21, and p27 expressions in control or C19orf10-overexpressed UMUC-3 cells. (C) Western blotting analysis of N-cadherin, Vimentin, Snail, Slug, β-catenin, and p-β-catenin expressions in control or C19orf10 siRNA-expressing SW780 and J82 cells. (D) Western blotting analysis of N-cadherin, Vimentin, Snail, Slug, β-catenin, and p-β-catenin in control or C19orf10-overexpressed UMUC-3 cells.

Article Snippet: The following cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA): human BC cell lines SW780 (ATCC® CRL-2169), UMUC3 (ATCC® CRL-1749), 5637 (ATCC® HTB-9), T24 (ATCC® HTB-4), TCCSUP (ATCC® HTB-5TM), and J82 (ATCC® HTB-1), and the immortalized urothelial cell line SV-HUC-1 (ATCC® CRL-9520).

Techniques: Western Blot, Control, Expressing